WebMouse IgGκ light chain binding protein (m-IgGκ BP) conjugated to fluorescein isothiocyanate (FITC) is suitable for binding to mouse IgGκ light chain immunoglobulins, comprising a vast majority of mouse monoclonal primary antibodies; not suitable for use with mouse monoclonal IgGλ light chain primary antibodies. WebSep 1, 2024 · Because FITC binding to BCR confers the target B cells with immune-regulatory properties, our data indirectly suggest that Bregs (at least defined by the …
Fluorescent Dyes Science Lab Leica Microsystems
WebDec 11, 2024 · Fluorescence-based assays are extremely diverse, sensitive and robust experimental methods for investigating the conformational changes, enzyme kinetics, dynamics and molecular interactions. A prerequisite for most of these experimental approaches is to label the protein of interest with one or more extrinsic fluorophores with … WebApr 13, 2024 · Aptamer-based biosensors recognize target analytes via designed aptamers with high binding affinity and specificity and then convert biomolecular recognition into measurable physical or electrical signals via a readout component. Nanoparticles ... Subsequently, AuNP and FITC were connected by a single aptamer sequence after the … meloxicam controlled drug
Annexin V binding assay as a tool to measure apoptosis in ...
WebNegative control cells were incubated with FITC-dextran particles at a temperature of 4°C. After the specified time the cells were washed in PBS without Ca2 + and Mg2 + for 5 min at 700 xg. Cell... WebImmobilization of Antibodies/Proteins. Incubate the beads and biotinylated antibodies in PBS for 30 mins at room temperature using gentle rotation. Separate the antibody-coated beads with a magnet for 2–3 mins. Wash the coated beads 4–5 times in PBS containing 0.1% BSA. WebFITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. meloxicam cross reactivity